Pdx1-creer
WebPDX1 (pancreatic and duodenal homeobox 1), also known as insulin promoter factor 1, is a transcription factor in the ParaHox gene cluster. In vertebrates, Pdx1 is necessary for … Web037551 - NOD.pdx1 [PB] CreER [TM] Strain Details. Technical Information Scientists. Overview.
Pdx1-creer
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WebOct 22, 2010 · both Pdx1-CreER and RIP-CreER mice were generated using the. CreER. TM. variant, the tighter phenotype of the former is. conceivably due to the lower activity of the … WebMar 21, 2024 · PDX1 (Pancreatic And Duodenal Homeobox 1) is a Protein Coding gene. Diseases associated with PDX1 include Maturity-Onset Diabetes Of The Young, Type 4 and Pancreatic Agenesis 1. Among its …
WebJul 2, 2013 · The tamoxifen-inducible Tg (Pdx1-cre/Esr1 ∗) 1Mga line (also known as Pdx1PB -CreER) allows spatial and temporal control of gene manipulation specifically in … WebPdx1 Cre-ER (DM#255) was a gift from Douglas Melton (Addgene plasmid # 15022 ; http://n2t.net/addgene:15022 ; RRID:Addgene_15022) For your References section: Direct …
WebJun 15, 2010 · We utilized the Pdx1 promoter to misexpress Nkx6.1 or Nkx6.2 ( Figures 2 A and 2H ). As predicted, Pdx1-Nkx6.1 or Pdx1-Nkx6.2 embryos expressed Nkx6.1 or Nkx6.2, respectively, throughout the entire pancreatic epithelium, including the presumptive tip domain ( Figures 2 C, 2E, 2G, 2J, 2L, and 2N). WebJan 7, 2024 · First, p5 and p75 were used to amplify a 400 base pair fragment from the Pdx1 genomic DNA. Then this fragment was used together with p5 and p71 and the Cre-ER(TM) …
WebApr 4, 2024 · Pdx1 is a transcription factor required for the formation of endocrine pancreas that is inhibited during Coxsackievirus B4 infection of human pancreas ductal cells; recessive PDX1 mutations are a rare but important cause of isolated permanent neonatal diabetes in patients without pancreatic hypoplasia/agenesis
WebOct 21, 2024 · The basal unfasted morning glucose level of tamoxifen-treated Pdx1-CreER;Wnt4 fl/fl mice (Wnt4 βKO) was also high at 7 weeks, 2 months and 7 months (Fig. 3e–g). means function クレームWebThe focus will be on the CreER(T2) recombinase, which is currently the most successful CreER version. We will give an overview of available CreER(T2) transgenic mouse lines and present protocols that detail the generation of experimental mice for inducible gene knockout studies, the induction of recombination by tamoxifen treatment, and the ... peek footprint anchorWebDec 1, 2003 · To determine whether these early Pdx1 + cells represent a unique population with respect to Notch responsiveness, we used a transgenic strain, Pdx1-CreER , in which … means friend of the courtWebThe F1 progeny were heterozygous for Prmt5 (Prmt5+/l). Subsequent breeding between F1 mice resulted in a population of progeny that included the desired genotype Prmt5l/l. Then, Pdx1-CreER; Prmt5l/l mice were generated by intercrossing Pdx1-CreER mice with Prmt5l/l mice. (B-C) The genotypes were verified by PCR using tail DNA. peek foreachWebNov 28, 2008 · The β-cell–to-acinar transdifferentiation was observed not only when the caAkt mutant was expressed in β-cells during development (Rip-cre;caAkt), but also in mature β-cells from 4-week-old mice (Pdx1 CreER;caAkt). These results also demonstrate the plasticity of postnatal mature β-cells. means go or yieldWebthe lack of a d-cell-specific Cre line, we used Pdx1-CreER (Gu et al. 2002) mice, in which Cre recombinase is active in both b and d cells upon induction by tamoxifen (Guz et al. 1995; Serup et al. 1995; Schaffer et al. 2011). We reasoned that because Hhex is not expressed in b cells, 2014 Zhang et al. This article is distributed exclusively by ... peek frean \u0026 coWebThe unbridled insulin secretion in mutants stemmed from Hhex deficiency rather than ectopic expression of Cre in the β cell from the Pdx1-CreER transgenes, whose lack of effect on insulin secretion has been validated in several reports (Lee et al. 2011; Schaffer et al. 2011; Gao et al. 2014). peek flooring frisco tx